Fig. 2. Effect of palmitate on MIP-1α production in macrophages. THP-1 cells derived macrophages were treated with palmitate, TNF-a or BSA for 24 hrs. Cells and culture media were collected. RNA was isolated and MIP-1α mRNA was quantified by real time PCR (A). MIP-1α protein in culture media was determined by ELISA (B). Data are represented as mean ±SEM. Statistical analysis was done using t-test. P<0.05 was considered as statistically significant (* P<0.01; **P< 0.001, ***P< 0.0001).